International Journal of Infection

Published by: Kowsar

Optimization and Molecular Detection of Neuraminidase Gene in Listeria monocytogenes

Ciamak Ghazaei ORCID 1 , *
Author Information
1 Department of Microbiology, University of Mohaghegh Ardabili, Ardabil, Iran
Article information
  • International Journal of Infection: October 2018, 5 (4); e81884
  • Published Online: October 14, 2018
  • Article Type: Research Article
  • Received: July 8, 2018
  • Revised: October 3, 2018
  • Accepted: October 7, 2018
  • DOI: 10.5812/iji.81884

To Cite: Ghazaei C. Optimization and Molecular Detection of Neuraminidase Gene in Listeria monocytogenes, Int J Infect. 2018 ; 5(4):e81884. doi: 10.5812/iji.81884.

Copyright © 2018, International Journal of Infection. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License ( which permits copy and redistribute the material just in noncommercial usages, provided the original work is properly cited.
1. Background
2. Methods
3. Results and Discussion
4. Conclusions
  • 1. Gray MJ, Freitag NE, Boor KJ. How the bacterial pathogen Listeria monocytogenes mediates the switch from environmental Dr. Jekyll to pathogenic Mr. Hyde. Infect Immun. 2006;74(5):2505-12. doi: 10.1128/IAI.74.5.2505-2512.2006. [PubMed: 16622185]. [PubMed Central: PMC1459693].
  • 2. Hain T, Steinweg C, Chakraborty T. Comparative and functional genomics of Listeria spp. J Biotechnol. 2006;126(1):37-51. doi: 10.1016/j.jbiotec.2006.03.047. [PubMed: 16757050].
  • 3. Camilli A, Tilney LG, Portnoy DA. Dual roles of plcA in Listeria monocytogenes pathogenesis. Mol Microbiol. 1993;8(1):143-57. doi: 10.1111/j.1365-2958.1993.tb01211.x. [PubMed: 8388529]. [PubMed Central: PMC4836944].
  • 4. Liu D, Lawrence ML, Austin FW, Ainsworth AJ. A multiplex PCR for species- and virulence-specific determination of Listeria monocytogenes. J Microbiol Methods. 2007;71(2):133-40. doi: 10.1016/j.mimet.2007.08.007. [PubMed: 17884210].
  • 5. Joyce SA, Gahan CG. Molecular pathogenesis of Listeria monocytogenes in the alternative model host Galleria mellonella. Microbiology. 2010;156(Pt 11):3456-68. doi: 10.1099/mic.0.040782-0. [PubMed: 20688820].
  • 6. Vazquez-Boland JA, Kuhn M, Berche P, Chakraborty T, Dominguez-Bernal G, Goebel W, et al. Listeria pathogenesis and molecular virulence determinants. Clin Microbiol Rev. 2001;14(3):584-640. doi: 10.1128/CMR.14.3.584-640.2001. [PubMed: 11432815]. [PubMed Central: PMC88991].
  • 7. Schauer R, Kamerling JP. The chemistry and biology of trypanosomal trans-sialidases: Virulence factors in Chagas disease and sleeping sickness. Chembiochem. 2011;12(15):2246-64. doi: 10.1002/cbic.201100421. [PubMed: 21956798].
  • 8. Li J, Sayeed S, Robertson S, Chen J, McClane BA. Sialidases affect the host cell adherence and epsilon toxin-induced cytotoxicity of Clostridium perfringens type D strain CN3718. PLoS Pathog. 2011;7(12). e1002429. doi: 10.1371/journal.ppat.1002429. [PubMed: 22174687]. [PubMed Central: PMC3234242].
  • 9. Feng C, Li J, Snyder G, Huang W, Goldblum SE, Chen WH, et al. Antibody against microbial neuraminidases recognizes human sialidase 3 (NEU3): The neuraminidase/sialidase Superfamily Revisited. MBio. 2017;8(3). doi: 10.1128/mBio.00078-17. [PubMed: 28655817]. [PubMed Central: PMC5487728].
  • 10. Varki A, Schauer R. Chapter 14. Sialic acids. In: Varki A, Chrispeels MJ, editors. Essentials of glycobiology. 2nd. Cold Spring Harbor (New York): Cold Spring Harbor Laboratory Press; 2009.
  • 11. Lewis AL, Lewis WG. Host sialoglycans and bacterial sialidases: A mucosal perspective. Cell Microbiol. 2012;14(8):1174-82. doi: 10.1111/j.1462-5822.2012.01807.x. [PubMed: 22519819].
  • 12. Lovett J, Francis DW, Hunt JM. Listeria monocytogenes in raw milk: Detection, incidence, and pathogenicity. J Food Prot. 1987;50(3):188-92. doi: 10.4315/0362-028x-50.3.188.
  • 13. Jantzen MM, Navas J, Corujo A, Moreno R, Lopez V, Martinez-Suarez JV. Review. Specific detection of Listeria monocytogenes in foods using commercial methods: From chromogenic media to real-time PCR. Spanish J Agricult Res. 2006;4(3):235. doi: 10.5424/sjar/2006043-198.
  • 14. Khan JA, Rathore RS, Khan S, Ahmad I. In vitro detection of pathogenic Listeria monocytogenes from food sources by conventional, molecular and cell culture method. Braz J Microbiol. 2013;44(3):751-8. doi: 10.1590/S1517-83822013000300013. [PubMed: 24516442]. [PubMed Central: PMC3910184].
  • 15. Bradford MM. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem. 1976;72:248-54. doi: 10.1016/0003-2697(76)90527-3. [PubMed: 942051].
  • 16. Potier M, Mameli L, Belisle M, Dallaire L, Melancon SB. Fluorometric assay of neuraminidase with a sodium (4-methylumbelliferyl-alpha-D-N-acetylneuraminate) substrate. Anal Biochem. 1979;94(2):287-96. doi: 10.1016/0003-2697(79)90362-2. [PubMed: 464297].
  • 17. Wagner M, Auer B, Trittremmel C, Hein I, Schoder D. Survey on the Listeria contamination of ready-to-eat food products and household environments in Vienna, Austria. Zoonoses Public Health. 2007;54(1):16-22. doi: 10.1111/j.1863-2378.2007.00982.x. [PubMed: 17359442].
  • 18. Aygun O, Pehlivanlar S. Listeria spp. in the raw milk and dairy products in Antakya, Turkey. Food Control. 2006;17(8):676-9. doi: 10.1016/j.foodcont.2005.09.014.
  • 19. Dumen E, Baca AU. Comparative detection of Listeria monocytogenes in raw milk by microbiological method and PCR. Medycyna Weterynaryjna. 2008;64(1):59-63.
  • 20. Warwas ML, Watson JN, Bennet AJ, Moore MM. Structure and role of sialic acids on the surface of Aspergillus fumigatus conidiospores. Glycobiology. 2007;17(4):401-10. doi: 10.1093/glycob/cwl085. [PubMed: 17223648].
  • 21. Glomski IJ, Gedde MM, Tsang AW, Swanson JA, Portnoy DA. The Listeria monocytogenes hemolysin has an acidic pH optimum to compartmentalize activity and prevent damage to infected host cells. J Cell Biol. 2002;156(6):1029-38. doi: 10.1083/jcb.200201081. [PubMed: 11901168]. [PubMed Central: PMC2173464].
  • 22. Abrashev I, Dulguerova G. Neuraminidases (sialidases) from bacterial origin. Experiment Pathol Parasitol. 2001;4:35-40.
  • 23. Jost BH, Songer JG, Billington SJ. Cloning, expression, and characterization of a neuraminidase gene from Arcanobacterium pyogenes. Infect Immun. 2001;69(7):4430-7. doi: 10.1128/IAI.69.7.4430-4437.2001. [PubMed: 11401983]. [PubMed Central: PMC98516].
  • 24. Eneva R, Engibarov S, Strateva T, Abrashev R, Abrashev I. Biochemical studies on the production of neuraminidase by environmental isolates of Vibrio cholerae non-O1 from Bulgaria. Can J Microbiol. 2011;57(7):606-10. doi: 10.1139/W11-042. [PubMed: 21774613].
  • 25. Warwas ML, Yeung JH, Indurugalla D, Mooers AO, Bennet AJ, Moore MM. Cloning and characterization of a sialidase from the filamentous fungus, Aspergillus fumigatus. Glycoconj J. 2010;27(5):533-48. doi: 10.1007/s10719-010-9299-9. [PubMed: 20652740].
  • 26. Li J, McClane BA. The sialidases of clostridium perfringens type D strain CN3718 differ in their properties and sensitivities to inhibitors. Appl Environ Microbiol. 2014;80(5):1701-9. doi: 10.1128/AEM.03440-13. [PubMed: 24375134]. [PubMed Central: PMC3957610].

Featured Image:

Creative Commons License Except where otherwise noted, this work is licensed under Creative Commons Attribution Non Commercial 4.0 International License .
Readers' Comments