International Journal of Infection

Published by: Kowsar

Purification of 146s Antigen of Foot-and-Mouth Disease (FMD) Virus Serotypes Aby Using the Sucrose Gradient Procedure

Maryam Shojaee 1 and Saeid Zibaei 2 , *
Authors Information
1 Department of Biology, Payame Noor University of Mashhad, Mashhad, IR Iran
2 Razi Vaccine and Serum Research Institute, Agricultural Research, Education and Extension Organization (AREEO) Mashhad, IR Iran
Article information
  • International Journal of Infection: January 01, 2017, 4 (1); e41322
  • Published Online: August 30, 2016
  • Article Type: Research Article
  • Received: August 7, 2016
  • Accepted: August 11, 2016
  • DOI: 10.17795/iji-41322

To Cite: Shojaee M, Zibaei S. Purification of 146s Antigen of Foot-and-Mouth Disease (FMD) Virus Serotypes Aby Using the Sucrose Gradient Procedure, Int J Infect. 2017 ; 4(1):e41322. doi: 10.17795/iji-41322.

Copyright © 2016, Infectious Diseases and Tropical Medicine Research Center. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License ( which permits copy and redistribute the material just in noncommercial usages, provided the original work is properly cited.
1. Background
2. Objectives
3. Methods
4. Results
5. Discussion
  • 1. Arzt J, Juleff N, Zhang Z, Rodriguez LL. The pathogenesis of foot-and-mouth disease I: viral pathways in cattle. Transbound Emerg Dis. 2011; 58(4): 291-304[DOI][PubMed]
  • 2. Michel A, Rutten V. Transboundary and Emerging Diseases. 2013; 60
  • 3. Sutmoller P, Barteling SS, Olascoaga RC, Sumption KJ. Control and eradication of foot-and-mouth disease. Virus Res. 2003; 91(1): 101-44[PubMed]
  • 4. Grubman MJ, Baxt B. Foot-and-mouth disease. Clin Microbiol Rev. 2004; 17(2): 465-93[PubMed]
  • 5. Yoon SH, Park W, King DP, Kim H. Phylogenomics and molecular evolution of foot-and-mouth disease virus. Mol Cells. 2011; 31(5): 413-21[DOI][PubMed]
  • 6. Bachrach H. Foot and mouth disease virus: properties, molecular biology, and immunogenicity. 1977;
  • 7. Stave J, Card J, Morgan D, Vakharia V. Neutralization sites of type 01 foot-and-mouth disease virus defined by monoclonal antibodies and neutralization-escape virus variants. Virol. 1988; 162(1): 21-9
  • 8. Domingo E, Dávila M, Ortín J. Nucleotide sequence heterogeneity of the RNA from a natural population of foot-and-mouth-disease virus. Gene. 1980; 11(3-4): 333-46
  • 9. Martinez MA, Carrillo C, Plana J, Mascardla R, Bergada J, Palma EL. Genetic and immunogenic variations among closely related isolates of foot-and-mouth disease virus. Gene. 1988; 62(1): 75-84
  • 10. Sobrino F, Davila M, Ortin J, Domingo E. Multiple genetic variants arise in the course of replication of foot-and-mouth disease virus in cell culture. Virology. 1983; 128(2): 310-8[PubMed]
  • 11. Sobrino F, Palma EL, Beck E, Davila M, de la Torre JC, Negro P, et al. Fixation of mutations in the viral genome during an outbreak of foot-and-mouth disease: heterogeneity and rate variations. Gene. 1986; 50(1-3): 149-59[PubMed]
  • 12. Bachrach HL, Moore DM, McKercher PD, Polatnick J. Immune and antibody responses to an isolated capsid protein of foot-and-mouth disease virus. J Immunol. 1975; 115(6): 1636-41[PubMed]
  • 13. Bolwell C, Clarke BE, Parry NR, Ouldridge EJ, Brown F, Rowlands DJ. Epitope mapping of foot-and-mouth disease virus with neutralizing monoclonal antibodies. J Gen Virol. 1989; 70 ( Pt 1): 59-68[DOI][PubMed]
  • 14. Kleid DG, Yansura D, Small B, Dowbenko D, Moore DM, Grubman MJ, et al. Cloned viral protein vaccine for foot-and-mouth disease: responses in cattle and swine. Science. 1981; 214(4525): 1125-9[PubMed]
  • 15. Laporte J, Grosclaude J, Wantyghem J, Bernard S, Rouze P. Neutralisation en culture cellulaire du pouvoir infectieux du virus de la fièvre aphteuse par des sérums provenant de porcs immunisés a l'aide d'une protéine virale purifiée. CR Acad Sci Paris. 1973; 276(3399): 3
  • 16. Bittle JL, Houghten RA, Alexander H, Shinnick TM, Sutcliffe JG, Lerner RA, et al. Protection against foot-and-mouth disease by immunization with a chemically synthesized peptide predicted from the viral nucleotide sequence. Nature. 1982; 298(5869): 30-3[PubMed]
  • 17. DiMarchi R, Brooke G, Gale C, Cracknell V, Doel T, Mowat N. Protection of cattle against foot-and-mouth disease by a synthetic peptide. Science. 1986; 232(4750): 639-41[PubMed]
  • 18. Robertson B, Morgan D,, Moore D. Location of neutralizing epitopes defined by monoclonal antibodies generated against the outer capsid polypeptide, VP1, of foot-and-mouth disease virus A12. Virus Res. 1984; 1(6): 489-500[DOI]
  • 19. Strohmaier K, Franze R, Adam KH. Location and characterization of the antigenic portion of the FMDV immunizing protein. J Gen Virol. 1982; 59: 295-306[DOI][PubMed]
  • 20. Bachrach HL. Foot-and-mouth disease. Annu Rev Microbiol. 1968; 22: 201-44[DOI][PubMed]
  • 21. Reid SM, Hutchings GH, Ferris NP, De Clercq K. Diagnosis of foot-and-mouth disease by RT-PCR: evaluation of primers for serotypic characterisation of viral RNA in clinical samples. J Virol Methods. 1999; 83(1): 113-23
  • 22. Depa P, Dimri U, Sharma M, Tiwari R. Update on epidemiology and control of Foot and Mouth Disease-A menace to international trade and global animal enterprise. Veterinary World. 2012; 5(11): 694-704
  • 23. Garland A, Donaldson A. Foot-and-mouth disease. Surveillance. 1990; 17(4): 6-8
  • 24. Davies G. The foot and mouth disease (FMD) epidemic in the United Kingdom 2001. Compar Immunol Microbiol Infect Dis. 2002; 25(5): 331-43[DOI]
  • 25. Geering W. Foot‐and‐mouth disease in sheep. Australian Veterinary J. 1967; 43(11): 485-9[DOI]
  • 26. Booth JC, Rweyemamu MM, Pay TW. Dose-response relationships in a microneutralization test for foot-and-mouth disease viruses. J Hyg (Lond). 1978; 80(1): 31-42[PubMed]
  • 27. Cartwright B, Chapman WG, Brown F. Serological and immunological relations between the 146S and 12S particles of foot-and-mouth disease virus. J Gen Virol. 1980; 50(2): 369-75[DOI][PubMed]
  • 28. King AM, Newman JW. Temperature-sensitive mutants of foot-and-mouth disease virus with altered structural polypeptides. I. Identification by electrofocusing. J Virol. 1980; 34(1): 59-66[PubMed]
  • 29. Francis MJ, Fry CM, Rowlands DJ, Brown F. Qualitative and quantitative differences in the immune response to foot-and-mouth disease virus antigens and synthetic peptides. J Gen Virol. 1988; 69 ( Pt 10): 2483-91[DOI][PubMed]
  • 30. Collen T, McCullough KC, Doel TR. Induction of antibody to foot-and-mouth disease virus in presensitized mouse spleen cell cultures. J Virol. 1984; 52(2): 650-5[PubMed]
  • 31. Atsushi Shirai C, Chatchawanchonteera A, Sinsuwongwat W, Makarasen P, Chongming S. Estimation of 140S particles in foot-and-mouth disease virus (FMDV) vaccine by using the computer analyzing system. Japanese J Veterinary Sci. 1990; 52(3): 621-30[DOI]
  • 32. Aggarwal N, Barnett PV. Antigenic sites of foot-and-mouth disease virus (FMDV): an analysis of the specificities of anti-FMDV antibodies after vaccination of naturally susceptible host species. J Gen Virol. 2002; 83: 775-82[DOI][PubMed]
  • 33. Feng X, Ma JW, Sun SQ, Guo HC, Yang YM, Jin Y, et al. Quantitative Detection of the Foot-And-Mouth Disease Virus Serotype O 146S Antigen for Vaccine Production Using a Double-Antibody Sandwich ELISA and Nonlinear Standard Curves. PLoS One. 2016; 11(3): 149569[DOI][PubMed]
Creative Commons License Except where otherwise noted, this work is licensed under Creative Commons Attribution Non Commercial 4.0 International License .
Readers' Comments